在植物根毛中升的反应于Rhizobium结节信号
D W Ehrhardt1, R Wais, S R Long
1Howard Hughes Medical Institute, Department of Biological Sciences, Stanford University, California 94305-5020, USA.
Cell
|May 31, 1996
概括
豆 (Rhizobium lipochitooligosaccharide,LCO) 的信号分子会在豆类根毛中触发峰,这是结节发育的关键步骤. 缺乏这些峰的非结节突变者表明早期信号感知有缺陷.
科学领域:
- 植物与微生物的相互作用
- 植物中的分子信号传递.
- 豆类的共生关系是豆类的共生关系.
背景情况:
- 豆类共生涉及Rhizobium细菌形成固根结节.
- 来自Rhizobium的Lipochitooligosaccharide (LCO) 信号分子启动了这种共生.
- 对LCOs的精确植物信号传导途径在很大程度上是未知的.
研究的目的:
- 研究细胞质在植物对LCO信号分子的反应中的作用.
- 为了确定LCO诱导的刺是否对于结节是必不可少的.
- 为了检查一个非结节的突变的信号.
主要方法:
- 使用敏感的记者染料注入宿主根毛.
- 采用图像分析来检测和量化细胞质的波动.
- 评估了野生类型和突变的对LCOs的反应.
主要成果:
- 经过一段时间的延迟后,LCO的应用诱导了细胞质在根毛内局部的周期性峰值.
- 对于LCOs来引起结节反应的结构要求与尖的结构要求相同.
- 一种非结节的草突变体表现出完全没有LCO诱导的峰.
结论:
- 细胞质尖是豆类根毛对Rhizobium LCO信号的快速,早期反应.
- 尖是结节过程中必不可少的组成部分,可能参与信号感知.
- 非结节突变体在尖端的缺陷表明早期LCO信号感知或转导中的阻塞.
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