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相关概念视频

Plasmodesmata02:32

Plasmodesmata

30.0K
The organs in a multicellular organism’s body are made up of tissues formed by cells. To work together cohesively, cells must communicate. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
30.0K
Short-distance Transport of Resources02:12

Short-distance Transport of Resources

14.9K
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
14.9K
The Apoplast and Symplast01:46

The Apoplast and Symplast

51.4K
Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
51.4K
Animal and Plant Cell Structure01:30

Animal and Plant Cell Structure

31.5K
Animal and plant cells not only differ in their structure, function, and mode of nutrition but also in how they reproduce, specialize, and organize into complex structures.
Cell Division
Though both plant and animal cells divide by mitosis (for non-gametic cells) and meiosis (for gametic cells), they differ in the specifics of this process. Unlike animal cells, plant cells lack centrosomes — an organelle responsible for organizing the spindle fibers and segregating the chromosomes during...
31.5K
Plant Cell Wall01:07

Plant Cell Wall

7.7K
Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
7.7K
Plasmodesmata01:20

Plasmodesmata

3.8K
In a multicellular organism, cells must communicate to work together in a coordinated manner. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
Intercellular junctions are a feature of fungal, plant, and animal cells. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal cells include tight junctions, gap junctions, and...
3.8K

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相关实验视频

Updated: May 5, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
10:28

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Published on: October 19, 2018

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植物细胞包含两个功能上不同的真空体.

N Paris1, C M Stanley, R L Jones

  • 1Biochemistry Department, University of Missouri Columiba 65211, USA.

Cell
|May 17, 1996
PubMed
概括

植物细胞含有不同的真空单元,用于蛋白质储存和蛋白酶活性. 这些由α-TIP和TIP-Ma27标记的区块在真空孔成熟时合并,可能导致储存的蛋白质降解.

科学领域:

  • 植物细胞生物学 植物细胞生物学
  • 虚空的功能 虚空的功能
  • 贩卖蛋白质的人口贩运

背景情况:

  • 植物真空体具有多种功能,包括蛋白质储存和维护蛋白质酶活动的酸性pH值.
  • 以前,人们认为这些功能发生在单个真空腔区内.

研究的目的:

  • 为了研究植物细胞内真空功能的细分.
  • 为了确定不同的质质内在蛋白是否标记着不同的真空区.

主要方法:

  • 使用抗体对抗原体内在蛋白质 (α-TIP和TIP-Ma27) 的免疫标记.
  • 在真空区内追踪特定蛋白质 (大麦单蛋白和氨酸) 的定位.

主要成果:

  • 对α-TIP和TIP-Ma27的抗体标记着不同的真空膜区.
  • 麦莱克仅在α-TIP区内发现,而阿勒在TIP-Ma27区内发现.
  • 随着真空洞的成熟,这些不同的隔间合并.

结论:

  • 植物真空包括功能上不同的隔间,由特定的质质蛋白标记.
  • 在真空细胞发育过程中,这些区间的合并可能会促进储存的蛋白质的降解.

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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

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相关实验视频

Last Updated: May 5, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

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Visualizing Yeast Organelles with Fluorescent Protein Markers
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Visualizing Yeast Organelles with Fluorescent Protein Markers

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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

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  • 这一发现挑战了长期以来关于单一,均的真空区的假设.