人类单细胞-内皮细胞相互作用诱导颗粒细胞-巨细胞殖民地刺激因子的合成
M Takahashi1, S Kitagawa, J I Masuyama
1Department of Cardiology, Jichi Medical School, Togichi, Japan.
Circulation
|March 15, 1996
概括
单细胞和内皮细胞 (ECs) 之间的直接相互作用会诱导颗粒细胞-巨细胞殖民地刺激因子 (GM-CSF) 的产生. 这种局部化的GM-CSF通过影响单细胞/巨细胞功能,在动脉样硬化和炎症中发挥关键作用.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 病理生理学 病理生理学
背景情况:
- 单细胞对内皮的粘附是动脉样硬化和炎症的关键早期事件.
- 颗粒细胞-巨细胞殖民地刺激因子 (GM-CSF) 调节单细胞功能,包括粘附和细胞因子的产生.
研究的目的:
- 研究人类单细胞和人类静脉内皮细胞 (ECs) 之间的直接细胞对细胞相互作用是否诱导GM-CSF合成.
主要方法:
- 使用北方涂抹和ELISA对GM-CSFmRNA和蛋白质表达的分析.
- 单细胞和ECs的共同培养,有或没有中性粒细胞.
- 免疫组织化学和现场杂交以检测GM-CSF局部化.
- 使用抗GM-CSF抗体,基因斯坦和对IL-1和TNF-alpha的抗体进行抑制研究.
主要成果:
- 单细胞和ECs的共同培养显著提高了GM-CSFmRNA和蛋白质表达的调节.
- 转基因-CSF被分泌到超级生物中,刺激中性粒细胞超氧化物释放.
- 转基因CSF的产生局部化在附着单细胞和EC中,而不是非附着单细胞.
- 基因基因-CSF的产生受到基因斯坦的抑制,并且部分受到抗IL-1和抗TNF-alpha抗体的抑制.
结论:
- 单细胞和EC之间的直接相互作用诱导了GM-CSF的产生.
- 在单细胞-EC接口上局部产生的GM-CSF在动脉样硬化和炎症的发病过程中至关重要.
- 在体内,GM-CSF调节单细胞/巨细胞的功能,有助于炎症过程.
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