对GDNF多组分受体的表征
J J Treanor1, L Goodman, F de Sauvage
1Department of Neuroscience, Genentech, Inc., South San Francisco, California 94080, USA.
Nature
|July 4, 1996
概括
质细胞系衍生神经营养因子 (GDNF) 需要一种新型蛋白质GDNFR-alpha来产生其作用. 这种蛋白质与Ret受体形成复合体,启动信号通路,这对于神经元的生存和发育至关重要.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 质细胞系衍生神经营养因子 (GDNF) 对神经元的生存和发育至关重要.
- GDNF作用的确切机制在很大程度上是未知的.
- 在中枢和外周神经系统以及脏和肠道神经系统的发育中,GDNF起着至关重要的作用.
研究的目的:
- 阐明基质细胞系衍生神经营养因子 (GDNF) 信号的基础分子机制.
- 为了确定参与GDNF介导的生理反应的受体成分.
主要方法:
- 基质细胞系衍生神经营养因子 (GDNF) 结合相互作用的表征.
- 研究一种新型葡萄糖酸氨基醇 (GPI) 相关蛋白 (GDNFR-alpha) 在GDNF信号传递中的作用.
- 分析GDNFR-alpha与Ret受体氨酸激酶之间的物理关联.
主要成果:
- 一种新型的葡萄糖酸氨基醇 (GPI) 相关蛋白质GDNFR-alpha被确定为一种高亲和度的Glial细胞系衍生神经营养因子 (GDNF) 结合蛋白.
- GDNFR-α在响应GDNF的细胞上表达.
- 质细胞系衍生神经营养因子 (GDNF) 诱导GDNFR-alpha和孤儿氨酸激酶受体Ret之间形成复合物,导致Ret氨酸酸化.
结论:
- 质细胞系衍生神经变因子 (GDNF) 使用多个子单元受体复合体进行信号传递.
- GDNFR-alpha 起到连接体结合元件的作用,而 Ret 受体则起到信号转换元件的作用.
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