COP9综合体是一个新型的多子单元核调节器,涉及到工厂发展开关的光控制
D A Chamovitz1, N Wei, M T Osterlund
1Department of Biology, Yale University, New Haven, Connecticut 06520-8104, USA.
Cell
|July 12, 1996
概括
阿拉比多普西斯的COP9复合体通过调节COP1核丰度来调节光控制的发育. 这种保存的蛋白质复合体,包括COP11在内的12个子单元,对于植物光形生成至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 发育生物学是发展生物学.
- 生物化学 生物化学
背景情况:
- 光是调节植物发育的关键环境信号.
- COP9复合体 (也称为光形生成调节器) 对于调节光反应至关重要.
- 以前的研究表明COP9复合体在光形生成中的作用,但其确切的功能和组成尚不清楚.
研究的目的:
- 为了生物化学地表征阿拉比多普西斯COP9复合体.
- 确定COP9综合体的子单位及其潜在的角色.
- 阐明COP9复合体调节光形生成的机制.
主要方法:
- 生物化学净化COP9复合物,使其几乎均.
- 综合体内的12个不同的子单位的识别,包括COP11.
- 对突变表型和蛋白质与蛋白质相互作用的分析.
主要成果:
- COP9复合体是一种酸性,氨酸结合的核复合体,由12个子单位组成.
- COP11被确定为一个子单位,COP11的突变是cop9的突变.
- COP9复合体可能调节COP1的核丰度,这是光形生成的抑制剂.
- 对COP9综合体进行适当的核转移需要先前与子单位进行关联.
结论:
- COP9综合体是光控制植物发展的多个子单元调节器.
- COP11是COP9综合体的一个关键组成部分,对其功能至关重要.
- COP9复合体在调节COP1丰度中的作用是其在光形生成中的功能的关键.
- COP9复合体代表了高级真核生物中保存的发育调节器,COP9和COP11的人类同类.
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