编程细胞死亡的转录调节器由Caenorhabditis elegans基因ces-2编码
M M Metzstein1, M O Hengartner, N Tsung
1Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Nature
|August 8, 1996
概括
在C. elegans中,ces-2基因激活了被编程的细胞死亡. 它的蛋白质产物CES-2是一种类似于哺乳动物PAR蛋白的转录因子,表明它在细胞死亡途径中起着保留作用.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 在C. elegans中,细胞死亡规范 (ces) 基因调节了编程细胞死亡.
- 这些基因是进化控制细胞死亡的保存途径的候选者.
- 了解这些基因可以了解基本的生物过程.
研究的目的:
- 首次对一个 ces 基因进行分子特征鉴定.
- 为了研究 ces-2 基因在编程细胞死亡中的作用.
- 探索细胞死亡调节者的进化保护.
主要方法:
- 从C. elegans中克隆 ces-2 基因.
- 鉴定 ces-2 基因产物作为一个基本区域白-拉链 (bZIP) 转录因子.
- 对CES-2蛋白与已知的转录因子家族进行比较分析.
主要成果:
- 克隆了 ces-2 基因并进行了鉴定.
- ces-2对于激活特定的C. elegans神经元 (NSM姐妹细胞) 中的编程细胞死亡至关重要.
- 该CES-2蛋白与bZIP蛋白的PAR亚家族具有显著的相似性,包括DNA结合的特异性.
结论:
- ces-2基因编码了一个bZIP转录因子,该因子对C. elegans.中编程细胞死亡至关重要.
- 该CES-2蛋白与哺乳动物PAR蛋白的相似性表明,该家族在调节细胞死亡方面具有进化保守的作用.
- 这一发现支持了CES-2/PAR家族转录因子是跨物种编程细胞死亡的保守调节者的假设.
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