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Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection08:15

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

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We have developed a protocol for the generation and evaluation of a humanized and human immunodeficiency virus-infected NOG mouse model based on stem cell transplant, intravaginal human immunodeficiency virus exposure, and droplet digital PCR RNA...
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Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease09:24

Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease

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This paper introduces a method for repeated measurements of ventilation and respiratory muscle activity in a freely behaving amyotrophic lateral sclerosis (ALS) mouse model throughout disease progression with whole-body plethysmography and electromyography via an implanted telemetry...
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Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors05:46

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Here we describe cellular cytotoxicity and single round infectivity assays that allow for the rapid and accurate screening of compounds to determine their cellular cytotoxicity (CC50) and IC50 values against WT and drug resistant...
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Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays13:58

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

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Quantifying levels of HIV-1 RNA in plasma and sequencing single HIV-1 genomes from individuals with viral loads below the limit of detection (50-75 copies/ml) is difficult. Here we describe how to extract and quantify plasma viral RNA using a real time PCR assay that reliably measures HIV-1 RNA down to 0.3 copies/ml and how to amplify viral genomes by single genome sequencing, from samples with very low viral...
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Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V311:10

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

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HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5...
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Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy13:08

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

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This visualized experiment is a guide for utilizing a fluorescent molecular clone of HIV for live confocal imaging...
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相关实验视频

Updated: Jan 20, 2026

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
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与艾滋病毒相关的呼吸道疾病

R Miller1

  • 1Department of Sexually Transmitted Diseases, University College London Medical School, UK.

Lancet (London, England)
|August 3, 1996
PubMed
概括

艾滋病毒感染者经常出现肺部问题,包括机会性感染,如肺炎和细菌感染. 结核病和癌症,如卡波西 (Kaposi) 的癌症.

科学领域:

  • 肺部病理学 肺部病理学
  • 传染性疾病 传染性疾病
  • 在瘤学瘤学.

背景情况:

  • 艾滋病毒感染者经常患上机会性感染和影响肺部的瘤.
  • 超过三分之二的艾滋病毒患者至少经历过一次呼吸系统发作.
  • 尽管采取了预防措施,肺囊性肺炎仍然很常见.

研究的目的:

  • 审查艾滋病毒感染者常见的肺部并发症.
  • 突出艾滋病毒患者呼吸系统疾病的发病率和类型.

主要方法:

  • 对艾滋病毒患者的机会性感染,瘤和呼吸系统疾病的文献综述.
  • 对HIV肺部感染的流行病学数据的分析.

主要成果:

  • 肺囊炎肺炎是艾滋病毒患者常见的呼吸道疾病.
  • 细菌感染和结核病是致病和死亡的原因越来越多.
  • 卡波西肉瘤和非霍奇金淋巴瘤通常会影响艾滋病毒患者的肺部.

结论:

  • 肺部并发症是艾滋病毒管理中的一个重要问题.
  • 早期诊断和治疗呼吸系统疾病对艾滋病毒患者至关重要.

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  • 综合护理应针对HIV感染的传染性和瘤性肺部疾病.