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相关概念视频

Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

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相关实验视频

Updated: Jul 17, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

来自芽酵母的Cdk激活激酶 (CAK).

P Kaldis1, A Sutton, M J Solomon

  • 1Yale University School of Medicine, Department of Molecular Biophysics and Biochemistry, New Haven, Connecticut 06520-8024, USA.

Cell
|August 23, 1996
PubMed
概括

研究人员从酵母中净化和克隆了Cdk激活激酶 (CAK),发现它的功能与以前研究的CAK不同. 这一发现挑战了p40MO15-cyclin H-MAT1作为脊椎动物生理学CAK的作用.

科学领域:

  • 分子生物学分子生物学
  • 细胞循环规则 细胞循环规则
  • 生物化学 生物化学

背景情况:

  • 循环素依赖激酶 (CDK) 的激活对于细胞周期进展至关重要.
  • 这种激活需要与循环素和特定酸化的关联,特别是在人类p34cdc2.2.c161的氨酸残留物中.
  • Cdk激活激酶 (CAK) 负责这一重要的酸化事件.

研究的目的:

  • 为了净化和克隆来自Saccharomyces cerevisiae (酵母) 的CAK.
  • 描述酵母CAK (Cak1p) 的特性,并将其与其他生物体的CAK进行比较.
  • 通过使用遗传方法,研究CAK在细胞循环调节中的生理作用.

主要方法:

  • 从S. cerevisiae中净化和克隆CAK.
  • 酵母CAK在大肠杆菌中的表达,以评估其活性.
  • 在CAK1基因中创建和分析温度敏感突变.
  • 基因相互作用研究与CLB2基因,编码一个主要的线粒细胞循环.

主要成果:

  • 酵母CAK (Cak1p) 作为单体活跃,并在大肠杆菌表达时表现出完全活性.
  • 与其他物种的CAK不同,Cak1p不是基底转录因子TFIIH的一部分.

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Last Updated: Jul 17, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

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Published on: November 8, 2006

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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  • 在CAK1中对温度敏感的突变导致G2细胞周期延迟,减少Cdc28p激酶活性,以及与CLB2.2的遗传相互作用.

    • 结论:

    • 与其他生物体的CAK相比,酵母CAK (Cak1p) 具有独特的生化和功能性质.
    • 这些发现表明,以前在脊椎动物中发现的p40MO15-环林H-MAT1复合体可能不是主要的生理CAK.
    • 这项研究为了解不同物种中CDK激活的多样化机制开辟了新的途径.