Civ1 (CAK in vivo),是一种新型的Cdk激活激酶
J Y Thuret1, J G Valay, G Faye
1Service de Biochimie et Génétique Molécularie, Gif-sur-Yvette, France.
Cell
|August 23, 1996
概括
研究人员在酵母中发现了Civ1,一种新的Cdk激活激酶 (CAK). Civ1对于细胞活力至关重要,化Cdc28以调节细胞分裂和基因表达.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 循环素依赖蛋白激酶 (Cdks) 调节细胞分裂和基因表达.
- Cdk的激活通常需要通过Cdk激活激酶 (CAK) 结合环林和酸化.
研究的目的:
- 确定和描述Saccharomyces cerevisiae (S. cerevisiae) 中一种新的CAK活性.
- 为了研究这种新型CAK在体内生理学上的相关性.
主要方法:
- 序列相似性分析以确定潜在的CAK候选者.
- 生物化学测试以确认酶活性和基质特异性.
- 使用温度敏感突变体进行基因分析,以评估体内功能.
主要成果:
- 鉴定Civ1 (CAK in vivo),一种新的CAK,其序列与Cdks相似,但作为单体活跃.
- Civ1直接结合并化Cdc28,使其能够随后结合和激活环素.
- CIV1基因对酵母活力至关重要,而civ1-4突变体在Cdc28酸化和活性中表现出条件缺陷.
结论:
- Civ1是第一个被确定的CAK,具有遗传证据,具有重要的体内生理相关性.
- Civ1可能代表一种新酶家族的创始成员.
- 在S. cerevisiae中,Civ1在调节细胞分裂和基因表达方面发挥着关键作用.
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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
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In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
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