在小鼠中,前后模式的全球调节器的定位克隆
A Shumacher1, C Faust, T Magnuson
1Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Nature
|September 19, 1996
概括
胚胎外皮发育 (eed) 基因对于小鼠胚胎的前后模式至关重要. 缺食会破坏胃流动,导致严重的发育缺陷和改变轴骨架形成.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 前后 (A-P) 模式对于脊椎动物胚胎发育至关重要.
- 鼠三胸 (trx) 和多组 (Pc-G) 基因调节轴结构的 A-P 模式.
- 这些基因证明了Drosophila和小鼠之间的同源途径的上游调节.
研究的目的:
- 报告鼠标突变eed (胚胎外皮发育) 的定位克隆.
- 调查eed的作用,Drosophila Pc-G基因esc (额外的性别) 的同类在胚胎发育中的作用.
主要方法:
- 对eed突变的定位克隆.
- 在小鼠胚胎中对同卵性无基因基因的分析.
- 对小鼠胚胎中低形态ED突变的分析.
主要成果:
- 同卵性无卵性突变体在胃流动过程中显示出破坏的AP模式,缺少节点,记节,索米特和神经诱导.
- 突变胚胎表现出丰富的胚外间皮.
- 低形态基因突变导致轴骨架的后部转变.
结论:
- 在整个胚胎发生过程中,eed基因是A-P模式的全球要求.
- 它在建立胚胎极性和轴向发育方面发挥着至关重要的作用.
- 这项研究强调了PCG基因在发育模式中的保留功能.
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