人类冠状动脉样硬化中的氧化特异性表位不仅限于氧化低密度脂蛋白
K D O'Brien1, C E Alpers, J E Hokanson
1Division of Cardiology, School of Medicine, University of Washington, Seattle 98195-6422, USA. cardiac@u.washington.edu
Circulation
|September 15, 1996
概括
氧化低密度脂蛋白 (OxLDL) 抗体在动脉样硬化中检测到超出LDL的氧化蛋白. 这表明抗氧化剂可能通过除了仅仅防止LDL氧化之外的其他机制来保护动脉样硬化.
科学领域:
- 心血管研究研究心血管研究
- 免疫组织化学 免疫组织化学
- 氧化压力生物学 氧化压力生物学
背景情况:
- 以前的研究表明,氧化低密度脂蛋白 (OxLDL) 存在于动脉样硬化斑块中.
- 有限的研究检查了人类冠状动脉中LDL以外的蛋白质上的OxLDL表位.
研究的目的:
- 调查人类冠状动脉中OxLDL表位的流行情况.
- 为了确定OxLDL识别的表位是否存在于动脉样硬化斑块中的LDL以外的其他蛋白质上.
主要方法:
- 20名患者的87个动脉样硬化和51个非动脉样硬化冠状动脉段的免疫组织化学.
- 使用单克隆抗体向巨细胞,光滑肌细胞,阿波利波蛋白B和OxLDL.
- 在体外实验中评估了在氧化应激下对非LDL蛋白质的OxLDL表皮质形成.
主要成果:
- 氧化低密度脂蛋白 (OxLDL) 抗体染色在动脉样硬化部分显著更高.
- 发现与细胞相关的OxLDL表位体没有与细胞相关的Apolipoprotein B,表明非LDL蛋白的标.
- 氧化应激诱导了人类血清白蛋白和apo A-I上的OxLDL表位;抗氧化剂抑制了这种形成.
- 在无ApoB介质中的巨细胞也产生了OxLDL表位.
结论:
- 动脉样硬化涉及蛋白质的氧化修饰超出LDL,包括细胞相关蛋白质.
- 这些发现支持氧化蛋白在动脉样硬化中的作用.
- 动脉样硬化中的抗氧化作用可能涉及除了防止LDL氧化之外的机制.
更多相关视频
相关概念视频
01:38Inflammation
Overview
01:31Lipid-derived Compounds in the Human Body
Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various biological processes .
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
Fat-soluble Vitamins
Fat-soluble vitamins, including vitamins A, D, E, and K, are required in minimal quantities, but their deficiencies can lead to severely abnormal physiological conditions. For example, vitamin A deficiency can cause night blindness, dry skin, delayed...
01:30Coronary Artery Disease I: Introduction
Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
01:26Coronary Artery Disease II: Pathophysiology
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
01:26Coronary Artery Disease IV: Preventive Measures
Effective preventive measures for coronary artery disease (CAD) focus on controlling modifiable risk factors, including cholesterol abnormalities and lifestyle changes.Cholesterol ManagementFirst, the Mediterranean diet and the American Heart Association advocate for maintaining low-density lipoprotein (LDL) cholesterol levels below 100 mg/dL, with a more stringent recommendation of below 70 mg/dL for individuals at high risk. LDL cholesterol, often termed "bad cholesterol," can lead to the...
01:30Atherosclerosis I: Introduction
Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...