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相关概念视频

pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

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相关实验视频

Updated: Jul 23, 2026

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

全球蛋白RNA前体分子:红色素细胞中的生物合成和过程.

R N Bastos, H Aviv

    Cell
    |July 1, 1977
    PubMed
    概括

    研究人员使用脉冲追踪技术研究红细胞中的全球蛋白mRNA产生. 他们在核中发现了更大的前体分子,揭示了全球蛋白信使RNA生物合成的途径.

    科学领域:

    • 分子生物学分子生物学
    • 细胞生物学 细胞生物学
    • 生物化学 生物化学

    背景情况:

    • 全球蛋白mRNA对于红细胞功能至关重要.
    • 了解mRNA生物合成对于细胞过程至关重要.

    研究的目的:

    • 为了研究红状腺细胞中的全球蛋白mRNA的生物合成途径.
    • 为了识别参与全球蛋白mRNA形成的前体分子.

    主要方法:

    • 标记RNA与补充DNA的杂交.
    • 使用了一种脉冲追踪技术.
    • 在红色素细胞核和细胞质中分析了RNA物种.

    主要成果:

    • 检测到三种核RNA物种与全球蛋白mRNA共享序列.
    • 确定了两个较大的前体:一个27S (约. 5000核酸) 和一个15S (约. 1500个核酸) 的分子.
    • 27S物种缺乏多A序列,而15S和10S物种含有这些序列. 27S前体迅速降解,而10S细胞质mRNA积累起来.

    结论:

    • 提出了全球蛋白mRNA生物合成的模型.

    更多相关视频

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    Isolation and Characterization of RNA-Containing Exosomes

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    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
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    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

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    相关实验视频

    Last Updated: Jul 23, 2026

    Transcriptome Analysis of Single Cells
    07:27

    Transcriptome Analysis of Single Cells

    Published on: April 25, 2011

    Isolation and Characterization of RNA-Containing Exosomes
    09:43

    Isolation and Characterization of RNA-Containing Exosomes

    Published on: January 9, 2012

    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
    06:29

    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

    Published on: April 7, 2023

  • 该途径涉及大27S前体的转录,其次是分裂成15S中间体.
  • 进一步处理产生成熟的10S全球蛋白mRNA用于细胞质积累.