概括
通过平衡刺激和抑制因素,可以精确控制巨细胞等离子体激活剂分泌. 这种酶对于研究炎症和细胞调节是有用的.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 巨细胞在免疫反应和炎症中起着关键作用.
- 等离子原激活剂是一种由巨细胞分泌的酶,它对组织重塑和炎症有影响.
研究的目的:
- 调查控制等离子体激活剂合成和巨细胞分泌的调节机制.
- 探索调节这种酶的产生的潜力,以研究细胞调节现象.
主要方法:
- 利用培养的巨细胞研究酶生产.
- 应用各种刺激剂 (康卡纳瓦林A,福尔博米里斯酸盐) 和抑制剂 (葡萄皮质激素,线粒抑制剂,cAMP调节剂).
- 在不同条件下量化酶的产生和分泌细胞的比例.
主要成果:
- 血原激活剂分泌被康卡纳瓦林A和博酸显著诱导/刺激.
- 葡萄糖皮质类药物,线粒激素抑制剂和cAMP调节剂抑制了酶的产生.
- 酶生产和分泌水平在200倍的范围内调节,分泌细胞的比例从1-90%不等.
- 在相同的条件下,酶和酸酸酶的生产没有显示出可比的调制.
结论:
- 巨细胞等离子体激活剂的产生是通过相互作用的对抗性刺激来生理控制的.
- 这种酶是研究调节机制的宝贵工具,特别是在炎症的背景下.
更多相关视频
08:31FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation
Published on: May 24, 2018
8.1K
11:31Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex
Published on: February 25, 2022
2.2K
相关概念视频
Chemical Synapses
9.5K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
9.5K
G-Protein Gated Ion Channels
5.5K
GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
Sensory...
5.5K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
4.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.6K
Chemical Synapses
10.9K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
10.9K
