通过CAF-1和乙化组织基因组H3/H4的复合体进行核细胞组合
A Verreault1, P D Kaufman, R Kobayashi
1Cold Spring Harbor Laboratory, New York 11724, USA.
Cell
|October 4, 1996
概括
染色体组合因子1 (CAF-1) 亚单元p48与基因子相互作用,并参与DNA复制依赖的染色体组合. 这表明CAF-1是核细胞组合中的关键中间体,而p48在基因素代谢中具有更广泛的作用.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体生物学 染色体生物学
背景情况:
- 染色体组装因子1 (CAF-1) 对于依赖DNA复制的核细胞组装至关重要.
- CAF-1,p48的小子单元属于WD重复蛋白的保留子家族,在人类和酵母中都有同类.
- p48还与基因素脱乙酶活性和染色体组合复合体有关.
研究的目的:
- 研究CAF-1 p48亚单元在染色体组合中的作用.
- 描述染色体组合复合体 (CAC) 的组成和功能.
- 探索p48在CAF-1之外的基因组代谢中的参与.
主要方法:
- 染色体组合复合体 (CAC) 的生物化学净化.
- 在CAC中分析基因组H4乙化模式.
- 研究p48与海斯H4的结合.
主要成果:
- 人类的p48可以独立于其他CAF-1子单元结合素H4.
- 含有CAF-1亚单元和H3/H4基因组的CAC促进了依赖于复制的染色体组合.
- CAC 基因组 H4 显示了一个独特的氨酸乙化模式,与新合成的 H4.4 区别开来.
结论:
- 在 de novo 核细胞组合中,CAC 是一个关键的中间体.
- 48子单元在染色质组合和潜在的其他与基因素相关的过程中发挥着重要作用.
- CAF-1及其p48亚单元对于理解DNA复制和基因素代谢至关重要.
相关概念视频
02:33The Nucleosome
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
02:10The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
02:30Histone Variants at the Centromere
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
02:33The Nucleosome
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
01:19The Nucleosome
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
01:12The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...