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人类TAFII 105是一个细胞类型特定的TFIID子单元,与hTAFII130相关

R Dikstein¹, S Zhou, R Tjian

¹Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.

|October 4, 1996

概括

研究人员发现了一种新的TFIID蛋白子单元,TAFII105,特别是在B细胞中. 这种特定于B细胞的TFIID组件可以调节这些细胞中的基因转录.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

TFIID复合体是所有细胞中至关重要的转录因子.
TAF子单位是核心 TFIID 综合体的重要组成部分.

研究的目的:

在差异化B细胞中识别新的TFIID子单位.
描述B细胞中新发现的TAF亚单元的功能和表达.

主要方法:

从不同细胞类型中分离和描述TFIID复合体.
新型TAF子单元的cDNA克隆和序列分析.
对TAFII105mRNA和蛋白质表达水平的分析.
使用短暂过度表达和基因转录试验的功能研究.

主要成果:

在分化的B细胞中发现了一种新型的105kDaTAF亚单元 (hTAFII105),但在其他细胞类型中没有发现.
hTAFII105与其他TAF共享一个保留的C端域,但具有不同的N端域.
虽然TAFII105mRNA被广泛表达,但该蛋白主要与B细胞中的TFIID相关.
过度表达hTAFII105可以选择性地抑制B细胞中某些基因的转录.

结论:

TAFII105是TFIID复合体的一个B细胞特异子单元.
TAFII105可能在B细胞中通过特定的激活剂调解转录中发挥作用.
这一发现突显了细胞类型特异性的转录因子组成和功能的调节.

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