梅纳是VASP和Drosophila Enabled的亲属,与控制微纤维动力学有关
F B Gertler1, K Niebuhr, M Reinhard
1Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Cell
|October 18, 1996
概括
研究人员确定了Mena和Evl,这两种蛋白与Drosophila Enabled相关,对于细胞运动性和行为蛋白聚合是至关重要的. 这些蛋白质可能在细菌的运动和细胞结构的形成中发挥作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- Drosophila Enabled对于轴突结构的形成至关重要,并与信号通路有关.
- 启用,血管扩散刺激蛋白 (VASP),Mena和Evl是涉及细胞骨调节的相关蛋白质.
研究的目的:
- 为了识别和表征Drosophila的小鼠同类. 启用.
- 调查Mena和Evl在actin动态和细胞运动中的功能.
主要方法:
- 蛋白质同质性分析以确定Mena和Evl.
- 生物化学测试以确定Mena与富含proline的动机和G-actin结合蛋白的相互作用.
- 基于细胞的测试使用表达神经丰富Mena异型的纤维细胞.
主要成果:
- 确定了Mena和Evl是与Drosophila Enabled和VASP高度相关的小鼠蛋白质.
- 在Mena中,一个保留的域将其定位为具有特定普罗林丰富基因的蛋白质.
- 梅纳与Listeria monocytogenes和profilin有关,这表明它在细菌运动的actin聚合中发挥了作用.
- 纤维细胞中Mena异型体的表达诱导了异常的富含F-actin的外生,表明它在微细丝组装中的作用.
结论:
- 梅纳和埃维尔是Drosophila Enabled的功能同类物.
- 梅纳在行为动力学,细胞移动性和潜在的细菌运动中发挥着重要作用.
- 这些发现突显了Enabled家族蛋白质在调节actin细胞骨架中的保留功能.
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