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相关概念视频

Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.

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相关实验视频

Updated: May 8, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 22, 2010

在Xenopus中,原产地识别复合体和复制许可系统之间的相互作用.

A Rowles1, J P Chong, L Brown

  • 1Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, United Kingdom.

Cell
|October 18, 1996
PubMed
概括

原始识别复合体 (ORC) 对于DNA复制的启动至关重要. 在Xenopus中,XORC1的枯竭阻止了复制,ORC与染色质的结合对于许可复制起源至关重要.

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • 原始识别复合体 (ORC) 是真核生物中DNA复制启动的关键调节器.
  • 了解不同物种的ORC功能对于理解细胞循环控制至关重要.

研究的目的:

  • 为了克隆和描述ORC (XORC1) 的Xenopus同类物 (Xenopus homolog).
  • 调查XORC1和Xenopus ORC (XORC) 在Xenopus蛋提取物中DNA复制启动和许可中的作用.

主要方法:

  • 对XORC1.1进行克隆.
  • 使用Xenopus蛋提取物进行免疫减弱测试.
  • 净化XORC. 的净化.
  • 在整个细胞周期中进行染色体结合测试.

主要成果:

  • 克塞诺普斯XORC1被克隆,其耗尽抑制了DNA复制的启动.
  • 纯化XORC类似于酵母ORC,并在G1和S阶段结合染色素.
  • 作为许可因素RLF-M的染色体关联取决于ORC,使启动蛋白的顺序组装成为可能.

结论:

更多相关视频

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

相关实验视频

Last Updated: May 8, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 22, 2010

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

  • 在Xenopus中,XORC在启动DNA复制方面发挥着至关重要的作用.
  • 对染色质的ORC结合是组装复制许可机制的先决条件.
  • 这项研究阐明了DNA复制起源的细胞周期依赖调节的一个关键步骤.