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修补的瘤抑制基因编码了Sonic hedgehog的候选受体
D M Stone1, M Hynes, M Armanini
1Department of Neuroscience, Genentech, Inc., South San Francisco, California 94080, USA.
Nature
|November 14, 1996
概括
声波刺 (Shh) 蛋白与瘤抑制剂Patched (Ptc) 结合. 这是一种瘤抑制剂. 补丁与Smo相互作用,表明Ptc在脊椎动物发育中充当Shh受体.
科学领域:
- 发展生物学 发展生物学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 声波刺 (Shh) 是一种关键的蛋白质,调节脊椎动物的发育,模式和生长.
- 补丁 (Ptc) 是一种已知的瘤抑制蛋白质,涉及到基底细胞癌.
- 滑化 (Smo) 是发育信号通路中的关键蛋白质.
研究的目的:
- 为了研究Sonic hedgehog (Shh) 和Patched (Ptc) 之间的相互作用.
- 在脊椎动物中探索修补 (Ptc) 和光滑 (vSmo) 之间的关系.
- 阐明在发育过程中SHH信号的分子机制.
主要方法:
- 蛋白结合试验证明了Shh和Ptc之间的高亲和相互作用.
- 同免疫沉显示Ptc和vSmo.mo.之间的物理复合体形成.
- 表达式分析以确定vPtc和vSmo在各种组织中的同定位.
主要成果:
- 声波刺 (Shh) 蛋白与Patched (Ptc) 具有很高的亲和力.
- 脊椎动物Ptc与脊椎动物滑化同类物 (vSmo) 形成一个物理复合体.
- vSmo与vPtc共同表达,但没有直接结合Shh.
结论:
- 补丁 (Ptc) 作为Sonic hedgehog (Shh) 的受体起作用.
- 脊椎动物光滑化 (vSmo) 是与Ptc受体相关的信号组件.
- 这些发现支持了涉及Ptc和vSmo的Shh信号转导模型.
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