黑色素瘤细胞表达Fas(Apo-1/CD95) 连接体:对瘤免疫逃脱的含义
M Hahne1, D Rimoldi, M Schröter
1Institute of Biochemistry, University of Lausanne, CH-1066 Epalinges, Switzerland. jurg.tschopp@ib.unil.ch
概括
黑色素瘤细胞表达Fas连接体 (FasL),可以杀死免疫细胞. 这种FasL表达可能有助于瘤逃避免疫检测,有助于免疫特权和癌症进展.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 皮肤病学 皮肤病学
背景情况:
- 恶性黑色素瘤是导致皮肤癌死亡的重要原因.
- 黑色素瘤细胞表达Fas联结体 (FasL),这是一个参与亡的分子.
- 在转移性病变中,FasL+瘤细胞位于Fas表达性免疫细胞附近.
研究的目的:
- 为了研究Fas连接体 (FasL) 在黑色素瘤瘤生长和免疫逃避中的作用.
- 确定黑色素瘤细胞表达的FasL是否有助于免疫特权.
主要方法:
- 在体外研究:用Fas敏感的细胞化黑色素瘤细胞.
- 在体内研究:将FasL+小鼠黑色素瘤细胞注入小鼠,包括Fas缺乏 (lpr突变) 的小鼠.
- 与FasL表达相关的瘤形成和免疫细胞透的分析.
主要成果:
- 黑色素瘤细胞在Fas敏感点细胞中诱导了亡 in vitro.
- 在注射FasL+黑色素瘤细胞的野生型小鼠中,瘤发生速度很快.
- 瘤发生在Fas缺乏 (lpr突变) 的小鼠中被推迟,这表明FasL在免疫效应细胞杀死中发挥了作用.
- 在转移性病变中,观察到FasL+瘤细胞靠近Fas表达T细胞透.
结论:
- 黑色素瘤细胞表达的Fas连接体 (FasL) 可以诱导免疫细胞的亡.
- FasL有助于瘤的免疫特权,通过使免疫逃避.
- 向FasL可能是恶性黑色素瘤的治疗策略.
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