通过酸化来调节与细胞循环相关的多A) 聚合酶
D F Colgan1, K G Murthy, C Prives
1Department of Biological Science, Columbia University, New York 10027, USA.
Nature
|November 21, 1996
概括
聚A聚合酶 (PAP) 在细胞分裂过程中被成熟促进因子 (MPF) 酸化. 这种高酸化抑制了PAP活性,可能减少M相细胞中的mRNA和蛋白质合成.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞信使RNAs (mRNAs) 的多元A尾部对于翻译启动和mRNA稳定性至关重要.
- 调节多尾合成是基因表达的一个关键步骤,特别是在早期发育过程中.
研究的目的:
- 通过p34(cdc2) /cyclin B (成熟促进因子,MPF) 的酸化来研究聚聚合酶 (PAP) 活性的潜在调节.
主要方法:
- 使用MPF进行PAP的体内和体外酸化试验.
- 在Xenopus laevis卵细胞的中性成熟过程中分析PAP酸化状态,并在M阶段捕获了HeLa细胞.
- 纯化的PAP和从线粒细胞中分离出来的PAP的酶活性测定.
主要成果:
- 在体内和体外,PAP被MPF酸化.
- 在Xenopus卵细胞成熟过程中,PAP经历过过酸化,并在M阶段逮捕了HeLa细胞.
- 由MPF介导的高酸化显著降低了PAP活性,而来自线粒细胞的PAP显示出抑制活性.
结论:
- 通过MPF介导的PAP酸化可能是抑制其在M阶段活动的调节机制.
- 这种PAP的抑制可能有助于在M阶段观察到的多A) +RNA和蛋白质合成的减少.
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