相关实验视频
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07:40
Dissection and Staining of Drosophila Larval Ovaries
Published on: May 13, 2011
赛克林D2是一种对FSH敏感的基因,参与淋巴细胞增殖和瘤发生
P Sicinski1, J L Donaher, Y Geng
1Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, USA.
Nature
|December 5, 1996
概括
这项研究表明,环素D2对于小鼠的雌性生育能力和雄性生殖器官发育至关重要. 它的缺失导致不育和低可塑性丸,这对人类的卵巢和丸癌有影响.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- D型循环素 (D1,D2,D3) 调节哺乳动物细胞周期的G1阶段.
- 这些环林在繁殖组织中被多余地表达,这引发了关于它们独特作用的疑问.
研究的目的:
- 调查环素D2在哺乳动物发育和细胞增殖中的特定功能.
- 了解控制D型环林表达的调节途径.
主要方法:
- 在胚胎干细胞中准基因以创建缺乏循环素D2的小鼠.
- 对突变雄性和雌性生殖表型的分析.
- 通过卵泡刺激激素 (FSH) 在卵巢颗粒细胞中诱导cyclin D2的研究.
- 检查人类卵巢和丸瘤中的cyclin D2 mRNA水平.
主要成果:
- 缺乏环素D2的雌性由于对FSH的反应而导致卵巢颗粒状细胞增殖受损,因此是无菌的.
- 突变雄性表现出低可塑性的丸.
- 环素D2是通过粒状细胞中的循环-AMP依赖途径由FSH特别诱导的.
- 在一些人类的卵巢和丸瘤中发现了升高的环林D2mRNA水平.
结论:
- 环素D2在哺乳动物生殖器官发育中起着关键的,非冗余的作用.
- 显著的细胞内信号通路调节不同D型环林的表达.
- 环素D2可能与人类某些卵巢和丸癌症的发展有关.
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