相关实验视频
Updated: Jul 21, 2026

12:23
Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
Published on: May 25, 2010
网林-1在脊椎动物神经系统的发育过程中,是指导 commissural 轴突所必需的
T Serafini1, S A Colamarino, E D Leonardo
1Howard Hughes Medical Institute, Department of Anatomy, University of California, San Francisco 94143-0452, USA.
Cell
|December 13, 1996
概括
在神经系统发育过程中,Netrin-1引导脊柱轴突突出. 这项研究证实了netrin-1的存在.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 脊柱 commissural 轴突向地板细胞导航,而三角膜运动轴突向外投射.
- 在实验室中,Netrin-1是一种来自地板细胞的蛋白质,它吸引脊柱轴突,并排斥三轴突.
- 网林-1在轴突指导中的体内作用在很大程度上仍未被描述.
研究的目的:
- 为了研究在神经系统发育过程中指导神经元投射中的netrin-1的体内功能.
- 为了确定网林-1是否在指导脊柱 commissural 和 trochlear 运动轴突方面发挥作用.
- 为了确定与轴突路径发现有关的潜在的额外指导线索.
主要方法:
- 利用缺少网林-1的小鼠观察轴突投射表型.
- 在突变小鼠中分析了脊柱辅管轴突突出.
- 检查了前脑沟的形成和三脑轴突路径的发现.
主要成果:
- 缺少Netrin-1的小鼠在脊柱 commissural轴突突出的显著缺陷,支持其作为指导线索的作用.
- 缺少netrin-1也导致了几个前脑部部位的缺陷.
- 喉轴突突出在很大程度上是正常的,表明存在其他引导机制.
结论:
- 在体内,Netrin-1是脊柱 commissural轴突的关键指导线索.
- 网林-1还有助于指导前脑的指导.
- 地板细胞产生额外的线索,包括三角膜轴突的独特化学排斥剂,突出显示一个协作指导系统.
更多相关视频
相关概念视频
Neurulation
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...
Determination
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Microtubules in Signaling
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

