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p62(dok):在慢性骨髓性白血病的原始细胞中,是一种构成性地氨酸酸化的,与GAP相关的蛋白质
N Carpino1, D Wisniewski, A Strife
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Cell
|January 24, 1997
概括
慢性骨髓性白血病 (CML) 涉及异常的p210(bcr-abl) 蛋白. 研究人员确定了一种新的信号分子,p62(dok),它与GAP相关,并参与CML和受体氨酸激酶通路.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 细胞信号传递 细胞信号传递
背景情况:
- 慢性骨髓性白血病 (CML) 的特征是BCR-ABL融合蛋白,该蛋白具有高氨酸激酶活性.
- 在CML患者的造血细胞表现出与p120 ras GTPase激活蛋白 (GAP) 相关联的构成性铁酸化62kDa蛋白.
研究的目的:
- 为了净化和描述在BCR-ABL表达细胞中发现的新型62kDa蛋白质.
- 研究这种新型蛋白质在细胞信号通路中的作用,特别是与BCR-ABL和受体氨酸激酶有关的作用.
主要方法:
- 从表达p210的造血细胞系净化p62蛋白质 (bcr-abl).
- 分析与p120 ras GTPase激活蛋白 (GAP) 的蛋白质关联.
- 在c-Kit受体激活时对氨酸酸化状态的研究.
主要成果:
- 一种新型蛋白质,被指定为p62(dok),被净化并确定为信号分子.
- p62(dok) 与GAP相关,这种关联与其氨酸酸化有关.
- 在c-Kit受体激活后,p62(dok) 经历了快速的铁酸化.
结论:
- p62 (((dok) 是一种新的信号分子,涉及到CML的病变发生.
- p62(dok) 功能下游的受体氨酸激酶,如c-Kit.
- 这些发现表明p62 (((dok) 是与白血病和其他细胞过程相关的信号传导途径的关键组成部分.
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