控制系统性获得性耐药性的Arabidopsis NPR1基因编码了一种含有ankyrin重复的新型蛋白质
H Cao1, J Glazebrook, J D Clarke
1Department of Botany, Duke University, Durham, North Carolina 27708-1000, USA.
Cell
|January 10, 1997
概括
阿拉比多opsis NPR1基因对植物免疫力至关重要,控制系统性获得性耐药性 (SAR). NPR1编码了一种新型蛋白质,其氨酸重复对于激活防御基因和抵抗病原体至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 植物病理学 植物病理学
背景情况:
- 系统性获得性抵抗 (SAR) 是植物对病原体的重要防御机制.
- 阿拉比多普西斯NPR1基因在启动SAR中发挥着中心作用.
- NPR1突变体表现出受损的防御反应和对感染的易感性增加.
研究的目的:
- 为了克隆和表征阿拉比多普西斯NPR1基因.
- 阐明NPR1在植物免疫中的分子功能.
- 调查安基林重复在NPR1活动中的作用.
主要方法:
- 基于地图的克隆被用来隔离NPR1基因.
- 测序了NPR1基因,以确定突变和蛋白质结构.
- 在npr1突变体中进行了转基因补充实验.
主要成果:
- NPR1编码了一种新型蛋白质,它含有ankyrin重复.
- 一个ankyrin共识序列的突变损害了NPR1的功能.
- 与野生型NPR1的补充恢复了SAR诱导和病原体耐药性.
- 过度表达NPR1的转基因植物对P. syringae.表现出增强的耐药性.
结论:
- 在Arabidopsis中,NPR1对于SAR诱导和病原体耐药性至关重要.
- 安基林重复对于NPR1在植物免疫中的功能至关重要.
- NPR1在增强作物对疾病的抵抗力方面有潜在的应用.
相关概念视频
Defenses Against Pathogens and Herbivores
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
The Antiviral System of Bacteria and Archaea: CRISPR
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.


