碳氧酶E是一种受控的分泌途径分类受体:基因消灭导致Cpe (脂肪) 小鼠的内分泌功能障碍
1Section on Cellular Neurobiology, Laboratory of Developmental Neurobiology, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Cell
|January 10, 1997
概括
碳氧酶E (CPE) 作为内分泌细胞中调节的分泌蛋白的关键分类受体. 在突变小鼠中缺少它会导致蛋白质错位和内分泌疾病.
科学领域:
- 分子内分泌学分子内分泌学
- 细胞生物学 细胞生物学
- 神经内分泌学神经内分泌学
背景情况:
- 调节分泌通路 (RSP) 对于内分泌和神经内分泌细胞中荷尔蒙释放至关重要.
- 蛋白质分类成分泌颗粒是RSP的一个关键步骤,但其潜在机制尚未完全理解.
- 识别特定的分类受体对于理解细胞分泌至关重要.
研究的目的:
- 通过RSP识别负责将蛋白质向分泌颗粒的分类受体.
- 研究这种受体在内分泌细胞中蛋白质分类和分泌中的作用.
- 为了确定受体缺乏对内分泌功能的影响.
主要方法:
- 定位研究以确定 pituitary Golgi 和分泌颗粒膜中的分类受体.
- 结合性测试用于评估RSP蛋白与构成性分泌蛋白的受体的特异性.
- 在Cpe (脂肪) 突变小鼠模型中对蛋白质分类和分泌的分析,该模型缺乏已识别的受体.
主要成果:
- 与膜相关的碳氧酶E (CPE) 被确定为分类受体.
- CPE特别结合受调节的分泌通路蛋白,包括亲激素.
- 在Cpe (脂肪) 突变小鼠中,亲阿片梅拉诺科丁被错误地分离到构成性途径中,导致不受管制的分泌.
结论:
- CPE作为内分泌细胞中调节的分泌蛋白质的关键分类受体.
- 失去CPE功能会导致蛋白质分类受损和分泌不受调节.
- 在突变小鼠中,CPE缺乏导致多种内分泌疾病,包括高胰岛素血症和不孕症.
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