相关实验视频

Updated: Aug 3, 2026

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

在缺乏连接素的小鼠中女性不孕 37

A M Simon¹, D A Goodenough, E Li

¹Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA.

|February 6, 1997

概括

康尼辛37对于卵巢卵泡的发育和排卵至关重要. 缺少连素37的小鼠表现出卵细胞发育停止和排卵受损,突出显示了细胞间通信的重要性.

科学领域:

生殖生物学生殖生物学
细胞信号传输细胞信号传输
发育生物学发展生物学

背景情况:

卵巢卵泡的发育涉及复杂的细胞-细胞通信.
由连接体形成的间隙结,促进细胞间信号传输.
连接素在产卵和排卵中的特定作用尚未完全理解.

研究的目的:

研究连素37在卵巢卵泡发育和排卵中的作用.
确定连素37缺乏对卵细胞成熟和卵泡生长的影响.

主要方法:

使用了37缺乏Connexin的小鼠模型.
检查了卵巢卵泡形态和卵细胞发育.
评估排卵率和红色体的形成.

主要成果:

连素37存在于卵细胞和粒状细胞之间的间隙结处.
缺乏Connexin 37的小鼠缺乏成熟的Graafian毛囊,并无法排卵.
在缺陷小鼠中实现介质能力之前,卵细胞发育停止.
不恰当的紫色体在缺乏卵巢的卵巢中发展.

结论:

通过间隙结的细胞-细胞信号传递,特别是涉及连接素37,对于 oogenesis 是至关重要的.
康尼辛37对于卵泡发育和排卵所需的协调细胞相互作用至关重要.
干扰连xin 37 功能会导致严重的生殖缺陷.

更多相关视频

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Published on: July 7, 2023

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.