大麦Mlo基因:一种新的植物病原体耐药性的控制元素
R Büschges1, K Hollricher, R Panstruga
1The Sainsbury Laboratory, John Innes Centre, Norwich, United Kingdom.
Cell
|March 7, 1997
概括
在大麦中,递归的mlo等位基因通过改变Mlo蛋白来赋予对粉状菌的抵抗力. 丧失Mlo功能的原因是植物的防御,这表明它在细胞死亡和病原体反应中起着双重作用.
科学领域:
- 植物病理学 植物病理学
- 分子遗传学 分子遗传学
- 植物与微生物的相互作用
背景情况:
- 大麦Mlo locus控制了对粉的敏感性,粉是一种由Erysiphe graminis f. sp.引起的重要农业疾病. 一群人. 一群人.
- 突变诱导的衰退等位基因 (mlo) 赋予广泛的抗性,这表明Mlo基因产物在植物防御中起着至关重要的作用.
研究的目的:
- 为了分离和描述大麦Mlo基因.
- 阐明了Mlo介导的粉耐药性背后的分子机制.
主要方法:
- 使用定位克隆来分离Mlo基因.
- 对11种突变物诱导的mlo等位基因和敏感的内基因复合物的分析.
主要成果:
- 已经成功分离了Mlo基因.
- 在mlo等位基因中的突变导致了改变的Mlo蛋白序列.
- 敏感的重组剂表现出恢复的野生型Mlo序列.
- 预计Mlo蛋白 (60 kDa) 具有多个跨膜螺旋的膜.
结论:
- Mlo蛋白很可能起到叶子细胞死亡和病原体防御的负调节作用.
- 功能性Mlo蛋白缺失使植物对病原体攻击的反应能力提高,激活多个防御功能.
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