LEF-1,一个核因子协调信号输入从无翼和十足的信号输入
1Medical Research Council, Laboratory of Molecular Biology, Cambridge, United Kingdom.
Cell
|March 21, 1997
概括
转录因子LEF-1与Ultrabithorax基因增强剂结合,调节基因表达. LEF-1集成了无翼和双头的信号,用于精确控制Drosophila的发育.
科学领域:
- 发育生物学是发展生物学.
- 分子遗传学 分子遗传学
- 信号传导是指信号的传导方式.
背景情况:
- 无翅膀 (wg) 和十头 (dpp) 信号通路对于Drosophila的胚胎发育至关重要.
- 这些途径调节关键发育基因的表达,包括像Ultrabithorax (Ubx) 这样的同源基因.
研究的目的:
- 为了识别和描述Ultrabithorax中肠增强器内的最小无翼响应序列.
- 调查小鼠转录因子LEF-1在调解无翼信号和调节Ubx表达中的作用.
- 了解LEF-1如何集成多个信号输入以实现增强器活动.
主要方法:
- 在Ubx中肠增强器中识别了最小的无翼响应序列.
- 对LEF-1结合该序列的分析,该序列与 armadillo 在三元复合体中结合.
- 稳定转换试验用于评估LEF-1的转录刺激.
- 对LEF-1进行过度表达研究,以观察发育表型.
- 调查LEF-1对转录活动的dpp信号的依赖性.
主要成果:
- 在Ultrabithorax增强剂中定义了一个最小的无翼响应序列.
- 鼠类转录因子LEF-1与该序列结合,形成与甲鸟的三元复合体.
- 通过LEF-1进行的转录刺激依赖于甲鸟,需要十角的反应序列.
- 过度表达LEF-1模仿无翼过度刺激的表型,并绕过无翼信号的需要.
结论:
- 在调节Ultrabithorax表达的过程中,LEF-1充当了无翼和无脑的信号传递的关键调解者.
- LEF-1集成了多个位置信号来协调增强器活动.
- 在Drosophila发育过程中,LEF-1在组装多蛋白增强体复合体方面发挥着架构作用.
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