拼接因子BBP与mRNA前分支点序列UACUAACAC特有相互作用
J A Berglund1, K Chua, N Abovich
1Howard Hughes Medical Institute, Department of Biology and Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA.
Cell
|May 30, 1997
概括
分支点桥梁蛋白 (BBP) 与酵母前mRNA中的UACUAAC序列结合. 它的哺乳动物基因组,SF1,也结合了这个序列,这表明BBP在定义两个生物体的分支点中的作用.
科学领域:
- 分子生物学分子生物学
- 在RNA分离过程中.
- 蛋白质-RNA 相互作用
背景情况:
- 分枝点序列 (UACUAAC) 对于真核生物的mRNA前拼接至关重要.
- 已知酵母拼接因子BBP (分支点桥梁蛋白) 与分支点附近的前mRNA相互作用.
- 哺乳动物拼接因子SF1与酵母BBP相同,是E复合体的一部分.
研究的目的:
- 为了研究酵母BBP的RNA结合特性.
- 为了确定BBP是否特别识别UACUAAC分支点序列.
- 为了比较酵母BBP及其哺乳动物SF1在分支点识别中的功能.
主要方法:
- 生产了复合酵母BBP蛋白,并测试了与RNA序列的结合.
- 进行了RNA结合试验,以评估BBP对UACUAAC序列的特异性.
- 进行了BBP和SF1RNA结合能力的比较分析.
主要成果:
- 重组酵母BBP直接识别并与保存的UACUAAC分支点序列结合.
- 哺乳动物SF1 (mBBP) 也表现出对UACUAAC序列的特异性.
- 观察到分支点序列的保存以及BBP和mBBP之间的RNA结合的差异.
结论:
- 酵母BBP作为一个分支结合蛋白,直接识别UACUAAC序列.
- 哺乳动物SF1是BBP的功能性正方体,具有保留的分支点序列特异性.
- 在酵母和哺乳动物拼接中,BBP在定义分支点序列方面发挥着重要作用.
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