相关实验视频
Updated: Aug 7, 2026

14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
通过p300/CBP联合激活剂对p53进行结合和调制
N L Lill1, S R Grossman, D Ginsberg
1The Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Nature
|June 19, 1997
概括
病毒瘤蛋白通过干扰p300/CBP结合来破坏p53的功能. 这种相互作用对p53至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 病毒学 病毒学
背景情况:
- 腺病毒E1A和SV40大T抗原基蛋白与p300/CBP转录协活性剂相互作用.
- 这种相互作用与细胞转化机制有关.
- 在T抗原上有一个共享的结合区域,这表明p53和p300/CBP功能之间存在联系.
研究的目的:
- 调查p300/CBP和p53.3之间的直接相互作用.
- 确定这种相互作用对p53介导的细胞过程的功能后果.
- 阐明p300/CBP作为p53活性调解者的作用.
主要方法:
- 同免疫沉测试检测蛋白质与蛋白质相互作用.
- 免疫光显微镜用于评估亚细胞局部化.
- 记者基因测试测量促进体激活.
- 细胞周期分析和细胞亡试验用于评估功能结果.
主要成果:
- 在没有病毒coproteins的情况下,p300/CBP直接与p53结合.
- p300和p53在核中同位,形成一个稳定的DNA结合复合体.
- 腺病毒E1A破坏了p21和bax促进体的p53-介导的转录激活.
- E1A抑制了p53诱导的G1细胞循环停止和细胞亡.
结论:
- p300/CBP作为p53.3的转录适配器.
- p300/CBP调节了p53在G1细胞周期检查点控制和亡诱导中的作用.
- 病毒瘤蛋白对p300/p53复合物的破坏有助于细胞转化.
相关概念视频
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

