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Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
13:13

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

Published on: December 3, 2012

通过快速结/深蚀电子显微镜可视化NSF及其膜受体综合体的结构和构造变化.

P I Hanson1, R Roth, H Morisaki

  • 1Department of Pharmacology and HHMI, Yale University School of Medicine, New Haven, CT 06510, USA.

Cell
|August 8, 1997
PubMed
概括

对于膜融合至关重要的NSF ATPase形成一个空洞的圆柱体,其形状随着核酸结合而改变. 这种结构洞察力揭示了NSF如何与SNARE复合体相互作用以调解膜融合.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...