将Mcm蛋白加载到DNA复制原点上是由Cdc6p和CDKs调节的
Cell
|August 22, 1997
概括
细胞DNA复制起源是由启动蛋白调节的. Cdc6p和Mcm7p结合起源于G1,而Mcm7p结合取决于Cdc6p,这表明细胞周期控制了DNA复制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 在真核生物中,DNA复制起源只能在每个细胞周期中激活一次.
- 像Orc,Cdc6和Mcms这样的启动蛋白与调节复制原始点火有关.
- 在整个细胞周期中控制原始点火的精确机制尚未完全理解.
研究的目的:
- 调查启动蛋白与S. cerevisiae中的复制起源的细胞周期依赖的关联.
- 阐明Cdc6p和Mcm7p在调节DNA复制起源活动中的作用.
- 确定防止DNA重复复制的调节机制.
主要方法:
- 甲交叉连接被用来研究蛋白质-DNA相互作用 in vivo.
- 在不同细胞周期阶段 (G1,G2,S) 评估了Cdc6p和Mcm7p与复制起源的关联.
- 研究了子宫外Cdc6p表达对Mcm7p招募的影响.
主要成果:
- Cdc6p和Mcm7p特别与G1阶段的复制起源有关.
- Mcm7p与起源的关联取决于Cdc6p的存在.
- 宫外Cdc6p表达在G2期间允许起源关联,但没有招募Mcm7p.
结论:
- 复制原始点火是由至少两个不同的机制调节的.
- 在起源处的Cdc6p占用是MCM蛋白加载的先决条件.
- 在S,G2和M阶段,S和M阶段的循环素依赖激酶 (CDK) 的活性可以防止McM在S,G2和M阶段的加载,从而确保每细胞周期一次的DNA复制.
相关概念视频
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...