Wnt信号极化了早期的C. elegans芽细胞体,以区分内皮和中皮
C J Thorpe1, A Schlesinger, J C Carter
1Institute of Molecular Biology, University of Oregon, Eugene 97403, USA.
Cell
|August 22, 1997
概括
五个基因 (mom-1到mom-5) 对于C. elegans的内皮发育至关重要. 这种Wnt信号通路调节了早期胚胎芽细胞体中的细胞极性和基因表达.
科学领域:
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 在C. elegans的4细胞阶段的一个偏振信号指导EMS芽质体产生内皮.
- 控制这种不对称的细胞命运决定的分子机制尚未完全理解.
研究的目的:
- 为了识别C. elegans中EMS芽细胞体对内皮生产必不可少的基因.
- 阐明信号通路在早期胚胎发生过程中建立细胞极性中的作用.
主要方法:
- 向前遗传查以确定影响内皮发育的突变.
- 分析特定芽细胞 (P2信号细胞和EMS) 中的基因功能.
- 在突变胚胎中检查线粒状的方向.
主要成果:
- 在5个基因 (mom-1到mom-5) 中发现了16种突变,这些基因对EMS诱导的内皮产生有必要.
- -1,-2和-3在P2信号细胞中起作用,而-4则在EMS芽细胞中起作用.
- P2信号降低了EMS子细胞中HMG域蛋白POP-1的调节.
- mom-2编码了一个Wnt家族成员,这表明一个保存的信号通路.
- 突变破坏了线粒状的方向,这表明Wnt信号在早期芽细胞体的细胞骨极性中起着作用.
结论:
- 包括Wnt通路组成部分在内的MOM基因对于C. elegans的不对称内皮特异性至关重要.
- 由P2衍生信号介导的Wnt信号影响EMS谱系中的POP-1水平和细胞骨极性.
- 这一途径在胚胎早期发育期间影响多个母细胞体中的细胞极性.
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