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神经素是轴突化学排斥剂塞马福林III的受体

Z He¹, M Tessier-Lavigne

¹Howard Hughes Medical Institute, Department of Anatomy, University of California, San Francisco 94143-0452, USA.

|August 22, 1997

概括

崩素-1 (Sema III) 通过与神经皮林结合来排斥感觉轴突. 神经素作为一个受体,介导Sema III的作用.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
发展生物学发展生物学

背景情况:

轴指导对于神经系统发育至关重要.
塞马福林是关键的指导线索,塞马III作为化学排斥剂.
塞马III排斥的机制尚未完全理解.

研究的目的:

在感官神经元中识别Sema III结合蛋白.
阐明这些蛋白质在Sema III介导的轴突排斥中的作用.

主要方法:

表达式克隆以寻找Sema III结合蛋白.
使用胚胎大鼠感官神经元.
使用抗体对已识别的结合蛋白.

主要成果:

确定了神经素作为一种高亲和度的Sema III结合蛋白.
对神经素的抗体阻断了Sema III对轴突的排斥作用.
神经皮林抗体阻止了由Sema III引起的生长体崩.

结论:

神经皮林是Sema III的受体或受体复合体的一部分.
神经皮林调解Sema III对感觉轴突的排斥作用.
这一发现促进了对轴突引导机制的理解.

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