假定的人类端粒酶催化子单元基因hEST2在瘤细胞和永生过程中受到上调
M Meyerson1, C M Counter, E N Eaton
1Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, USA.
Cell
|August 22, 1997
概括
研究人员确定了人类的hEST2基因,该基因对端粒酶激活至关重要. 这个基因,这个基因.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
背景情况:
- 端粒酶对于端粒维护至关重要,但在正常的人体体细胞中通常被抑制.
- 端粒酶的重新激活是癌症发展和细胞不朽化的标志.
- 了解端粒酶的调节对于癌症研究至关重要.
研究的目的:
- 为了识别和描述负责端粒酶活性的人类基因.
- 研究该基因在细胞不朽化和瘤进展中的作用.
主要方法:
- 基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因
- 在各种人体组织,癌细胞系和培养细胞中分析hEST2基因表达.
- 在细胞永生和分化过程中hEST2表达水平与端粒酶活性的相关性.
主要成果:
- 克隆的人类基因hEST2显示出与端粒酶催化子单元的显著序列相似性.
- 在初级瘤,癌细胞系和端粒酶阳性组织中hEST2的表达很高.
- 在正常的体细胞和端粒酶阴性组织中,hEST2表达是无法检测的,其水平与永生和分化期间的端粒酶激活相关.
结论:
- hEST2基因编码了人类端粒酶复合物的组成部分.
- 诱导hEST2mRNA表达是细胞不朽化和瘤进展中端粒酶激活所需的关键事件.
- hEST2代表了癌症治疗中的潜在治疗标.
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