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蛋白质记忆是通过通过分子内伴侣介导的改变折叠来实现的
U P Shinde1, J J Liu, M Inouye
1Department of Biochemistry, Robert Wood Johnson Medical School-UMDNJ, Piscataway, New Jersey 08854, USA.
Nature
|October 23, 1997
概括
细素类蛋白质作为分子内部的陪伴者,指导蛋白酶域折叠. 陪伴者中的突变会产生一个超级生物.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 蛋白质折叠 蛋白质的折叠
背景情况:
- 潜素的蛋白作为一个分子内伴侣.
- 这种机制在 prokaryotic 和 eukaryotic 蛋白质中保存,包括 prohormone-convertases.
研究的目的:
- 调查细素的作用作为一个分子内部的陪伴者.
- 为了确定伴侣中的突变是否可以诱导改变的蛋白质构造.
主要方法:
- 潜素的局部导向突变发生 (Ile(-48)-to-Val).
- 分析酶动力学,氨基酸序列 (测序和质谱学),伴侣蛋白结合亲和力,二次结构,热稳定性和野生类型和突变性亚细素E的基质特异性.
主要成果:
- 一个突变的分子内伴侣诱导了subtilisin E.中的"改变"的酶活性构造.
- 尽管氨基酸序列相同,但改变和野生类型的亚氨基酸表现出明显的伴侣结合亲缘关系,次要结构,热稳定性和基质特异性.
- 改变后的细素显示,与非同类伴侣相比,对其同类伴侣具有4.5倍的亲和力.
结论:
- 一个相同的多可以折叠成不同的形状受到其分子内伴侣的突变的影响.
- 这种被称为"蛋白质记忆"的现象表明,折叠途径可以在蛋白质结构和功能上留下持久的印记.
- 蛋白质记忆可能对理解蛋白质折叠和错误折叠疾病有重大影响.
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