相关实验视频
Updated: May 5, 2026

08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
18.0K
伊卡帕B酶复合体 (IKK) 含有两个酶子单元,即IKKalpha和IKKbeta,它们对于伊卡帕B酸化和NF-kappaB激活是必不可少的
E Zandi1, D M Rothwarf, M Delhase
1Department of Pharmacology, University of California at San Diego, La Jolla 92093-0636, USA.
Cell
|November 5, 1997
概括
这项研究确定和描述IKKbeta,这是IkappaB激酶 (IKK) 复合体的第二个子单元. IKKalpha和IKKbeta都对IkappaB酸化和NF-kappaB激活至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 免疫学 免疫学 免疫学
背景情况:
- 伊卡帕B激酶 (IKK) 复合体调节NF-kappaB激活,这是免疫和炎症的关键途径.
- 之前的工作确定并克隆了IKKalpha,这是IKK复合物的子单元.
研究的目的:
- 为了分子克隆和表征IKKbeta,IKK复合体的第二个子单元.
- 阐明IKKbeta在功能性IKK复合体和NF-kappaB激活中的作用.
主要方法:
- 分子克隆IKKbeta. 的分子克隆.
- 生物化学描述IKKbeta及其与IKKalpha的相互作用.
- 评估IKKalpha和IKKbeta对IkappaB酸化和NF-kappaB激活的贡献.
主要成果:
- IKKbeta与IKKalpha具有结构上的相似之处,包括一种激酶域,氨酸拉链和螺旋环-螺旋形状.
- IKKalpha和IKKbeta相互作用,形成一个功能性的IKK复合体.
- IKKalpha和IKKbeta的催化活动对于IkappaB的酸化和NF-kappaB的激活都至关重要.
结论:
- IKKbeta是IKK综合体的一个关键组成部分,与IKKalpha一起工作.
- IKKalpha和IKKbeta之间的相互作用对于调节NF-kappaB信号通路至关重要.
相关概念视频
NF-κB-dependent Signaling Pathway
7.7K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.7K
Anaphase Promoting Complex
2.5K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.5K
MAPK Signaling Cascades
7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
The JAK-STAT Signaling Pathway
10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
10.2K
PI3K/mTOR/AKT Signaling Pathway
5.2K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.2K
Calmodulin-dependent Signaling
5.0K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.0K

