GRASP65是一种参与戈尔吉水箱堆叠的蛋白质
F A Barr1, M Puype, J Vandekerckhove
1Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Cell
|November 5, 1997
概括
N-乙基胺 (NEM) 向戈尔基重组堆叠蛋白 (GRASP65),防止在线粒分裂过程中重新组装戈尔基. GRASP65和GM130蛋白对于戈尔吉堆叠和囊泡对接至关重要,即使化.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 戈尔吉装置在线粒分裂过程中经历碎片化,水箱分散.
- 细胞分裂后戈尔吉堆的重组对于细胞功能至关重要.
- 参与高尔基结构和囊泡运输的蛋白质是理解线粒体高尔基动态的关键目标.
研究的目的:
- 为了识别N-乙烯胺胺 (NEM) 的分子标,这些标可以在线粒分裂过程中抑制高尔基重组.
- 描述在戈尔吉堆叠中识别的标蛋白的作用及其与其他戈尔吉蛋白的关系.
主要方法:
- 用NEM处理细胞和无细胞系统.
- 使用抗体和重组蛋白质进行抗体生产和功能测定.
- 同免疫沉用于研究蛋白质复合体.
- 在线粒状条件下蛋白质酸化的分析.
主要成果:
- 尼姆抑制了戈尔吉水箱在线虫分裂后重新组装成堆叠的结构.
- 一种65kDa的蛋白质,被指定为戈尔吉重组堆叠蛋白 (GRASP65),被确定为一个主要的NEM目标.
- 抗体和重组GRASP65在体外阻断了戈尔吉水槽堆叠.
- 参与囊泡对接的GM130被确定为一个小的NEM目标,并被发现与GRASP65.5复合.
- GRASP65和GM130仍然与戈尔吉膜结合,并在线粒分裂过程中被酸化.
结论:
- 格拉斯普65对于戈尔吉水箱的堆叠至关重要.
- GRASP65-GM130复合体在戈尔吉堆叠和囊泡对接中发挥作用.
- 在线粒分裂过程中GRASP65和GM130的酸化可能会导致Golgi结构和功能的破坏.
相关概念视频
Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
Coat Assembly and GTPases
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
Golgi Matrix Proteins
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...


