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在Xenopus中Xsox17alpha和-beta介导内皮形成

C Hudson¹, D Clements, R V Friday

¹Department of Biological Sciences, University of Warwick, Coventry, United Kingdom.

|November 18, 1997

概括

两个Xenopus Sox17基因,Xsox17alpha和Xsox17beta,对于内皮发育至关重要. 它们在早期的Xenopus胚胎中调解激素诱导的内皮分化,在正常内皮形成中发挥关键作用.

科学领域:

发育生物学是发展生物学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

Sox17蛋白质是参与小鼠内皮发育的转录因子.
了解其他物种中Sox17同类的作用,可以阐明保存的发育途径.

研究的目的:

为了隔离和表征Xenopus同类的小鼠Sox17.
研究这些Xenopus Sox17基因 (Xsox17alpha和Xsox17beta) 在内皮分化中的作用.

主要方法:

隔离了Xenopus Sox17α和β基因.
在Xenopus动物帽中进行了激素和FGF诱导实验.
使用mRNA注射进行子宫外基因表达研究.
过度表达一个主导负的Xsox17beta构造.
对内皮标记基因表达的分析.

主要成果:

Xsox17alpha和Xsox17beta在胃肠内皮中表达.
激素诱导动物帽子中的Xsox17表达,但FGF没有.
异位Xsox17表达会诱导内皮标记物,而内皮标记物则被主导负构造阻断.
主导负构造也阻止了激素诱导的内皮形成.

结论:

克塞诺普斯Sox17基因调解了激素诱导的内皮分化途径.
这些基因对于Xenopus胚胎的正常内皮分化至关重要.

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