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在IL-12介导的瘤排斥中对Valpha14NKT细胞的要求

J Cui¹, T Shin, T Kawano

¹Core Research for Evolutional Science and Technology (CREST) Project, Japan Science and Technology Corporation (JST), 1-8-1 Inohana, Chuo-ku, Chiba, Japan 260.

Science (New York, N.Y.)

|December 31, 1997

概括

自然杀伤性T (NKT) 细胞对于干白素-12 (IL-12) 拒绝瘤至关重要. 删除Jalpha281基因段会删除Valpha14 NKT细胞,从而阻止IL-12的产生.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

一个特定的淋巴细胞亚群,Valpha14自然杀手T (NKT) 细胞,表达NK1.1和一个独特的T细胞受体.
这些细胞是由特定的T细胞受体Valpha14-Jalpha281.1.表达的定义.

研究的目的:

研究Valpha14 NKT细胞在介导IL-12 (IL-12) 瘤排斥中的作用.
为了确定Valpha14NKT细胞是否是IL-12抗瘤作用的主要媒介.

主要方法:

产生缺乏Jalpha281基因段的小鼠,因此缺少Valpha14 NKT细胞.
在IL-12给药后对Valpha14NKT细胞缺乏的小鼠进行瘤排斥的评估.

主要成果:

缺少Jalpha281基因段的小鼠完全没有Valpha14 NKT细胞.
这些缺陷小鼠在用IL-12治疗时未能排斥瘤.
鉴定出IL-12是Valpha14NKT细胞的必不可少的激活剂,然后使用NK类机制来消除瘤.

结论:

瓦尔法14NKT细胞对于IL-12诱导的抗瘤免疫是必不可少的.
IL-12主要通过激活Valpha14NKT细胞来调解瘤排斥,而不仅仅通过传统的NK细胞或T细胞.
向Valpha14 NKT细胞代表了增强IL-12癌症疗法的潜在策略.

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