在Drosophila中独立的光感受性昼夜钟
J D Plautz1, M Kaneko, J C Hall
1Department of Cell Biology and National Science Foundation Center for Biological Timing, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Science (New York, N.Y.)
|December 31, 1997
概括
果虫组织含有自主昼夜钟,表明单个细胞可以独立调节自己的生物节奏. 这些时钟对光有反应,这表明新的光受体参与了信号传导.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环节律是内生生物过程,调节日常循环.
- 果Drosophila melanogaster是研究昼夜节律的关键模型生物.
- 了解昼夜钟的分子机制对于各种生物过程至关重要.
研究的目的:
- 为了调查drosophila的外围组织中昼夜钟的存在和功能.
- 为了确定这些外围时钟是否自主且对光有反应.
- 为了确定参与光介导时钟重置的潜在光受体.
主要方法:
- 使用的转基因Drosophila表达光酶或绿色光蛋白在周期基因促进者的控制下.
- 通过脑部,胸部和腹部组织的生物发光来监测昼夜节律.
- 暴露在光线下,以评估节律重置和光感受性质.
主要成果:
- 节奏生物发光在各种被探索的组织中被观察到,包括感觉毛发.
- 这些组织中的昼夜节律在暴露于光线下成功地被重置.
- 有证据表明,未确定的光受体参与了外围时钟的光学调节.
结论:
- 自主昼夜振荡器分布在Drosophila的整个身体中.
- 单个细胞具有维持独立的昼夜时钟的能力.
- 周围组织拥有功能性昼夜钟,对光线线索敏感.
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