在基因尺度上探索基因表达的代谢和遗传控制
J L DeRisi1, V R Iyer, P O Brown
1Department of Biochemistry, Stanford University School of Medicine, Howard Hughes Medical Institute, Stanford, CA 94305-5428, USA.
Science (New York, N.Y.)
|October 24, 1997
概括
这项研究使用DNA微阵列来绘制Saccharomyces cerevisiae在从发酵转化为呼吸的代谢转变期间的基因表达变化. 它揭示了对代谢重编程和基因功能的见解,展示了一个强大的全基因组方法.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 系统生物学 系统生物学
背景情况:
- 了解代谢变化对于酵母生物技术至关重要.
- 基因表达动态调节细胞适应.
- 在Saccharomyces cerevisiae中的二氧化转移涉及复杂的转录重编程.
研究的目的:
- 在Saccharomyces cerevisiae中从发酵到呼吸的代谢转变期间全面调查时间基因表达程序.
- 识别参与代谢重编程的基因并阐明以前未被描述的基因的功能.
- 评估转录调节器TUP1和YAP1对全基因组基因表达的影响.
主要方法:
- 使用了DNA微阵列,几乎代表了所有Saccharomyces cerevisiae基因.
- 在二氧化转移期间分析了时间基因表达特征.
- 研究的基因表达在TUP1被删除和YAP1.1过度表达后发生的变化.
主要成果:
- 已知代谢基因的表达特征为在二氧化变化期间的代谢重编程提供了洞察力.
- 非特征化的基因的表达模式表明了潜在的功能.
- 鉴定了受TUP1删除和YAP1过度表达影响的基因.
结论:
- DNA微阵列为全基因组的基因表达模式的探索提供了一个可行的和强大的工具.
- 这项研究提供了在酵母中关键代谢过渡期间基因表达动态的详细地图.
- 这些发现有助于理解酵母代谢和基因调节.
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