鸟类毛的基因表达识别了一种与脊椎动物细分和体质发生相关的分子钟
I Palmeirim1, D Henrique, D Ish-Horowicz
1Institut d'Embryologie Cellulaire et Moléculaire du Centre National de la Recherche Scientifique et du Collège de France, Nogent sur Marne.
Cell
|December 11, 1997
概括
研究人员确定了c-hairy1,这是一种对鸟类细分至关重要的基因. 它在对轴间皮中的节奏表达表明脊椎动物和无脊椎动物体内生殖的一种保存的发育时钟.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 抛向性介质体的细分成体体是脊椎动物发育中的一个基本过程.
- 了解背后的分子机制 somitogenesis 是解读发育过程的关键.
- 在Drosophila中毛的细分基因为研究细分提供了一个模型.
研究的目的:
- 为了识别和表征Drosophila细分基因的鸟类同类.
- 为了研究发育中的小胚胎中c-hairy1的表达模式和动态.
- 为了确定c-hairy1在体生成过程中的作用.
主要方法:
- 从鸟类物种中识别和克隆c-hairy1基因.
- 整体安装在位杂交以分析c-hairy1 mRNA表达模式.
- 对c-hairy1表达的时间分析以与索米特形成相关联.
- 研究波形表达模式的潜在机制.
主要成果:
- 鉴定和鉴定了c-hairy1,这是Drosophila hairy的鸟类同类.
- c-hairy1 mRNA在前体质质中表达周期波,周期约为90分钟,与索米特形成时间相匹配.
- 观察到的波源来自于协调的表达脉冲,而不是细胞运动或信号传播.
- 节奏性c-hairy1表达似乎是对轴性介质皮的自主性质.
结论:
- c-hairy1在对轴性中皮的细分时钟中起作用.
- 这些发现提供了对调节体质发生的发育时钟的分子证据.
- 这项研究支持无脊椎动物和脊椎动物之间的细分机制的保护.
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