人类疹病毒8种变体在肉骨组织中
L Di Alberti1, A Piattelli, L Artese
1PHLS Virus Reference Division, Central Public Health Laboratory, London, UK.
Lancet (London, England)
|December 24, 1997
概括
人类疹病毒8 (HHV-8) 的DNA序列被检测在肉组织中,与非肉组织不同. 类似于mycobacteria的序列在sarcoid淋巴结中更常见,但没有确定特定的物种.
科学领域:
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
- 免疫学 免疫学 免疫学
背景情况:
- 萨尔科伊多सिस的病因仍然未知,之前的研究涉及菌根菌.
- 这项研究调查了在肉发病组织中存在的人类疹病毒8 (HHV-8) 和菌根菌DNA的情况.
研究的目的:
- 为了确定HHV-8和真菌细菌DNA序列是否存在于肉病患者的组织中.
- 为了比较这些序列在肉病组织中的流行率与非肉组织中的流行率.
主要方法:
- 分析了来自17名肉病患者和96名对照组的活检样本.
- 嵌套和半嵌套PCR技术被用于检测HHV-8 (ORF 26和ORF 25) 和菌根16SrRNA基因序列.
- 使用单链形态多态和二氧化序列测序分析了序列变异.
主要成果:
- 与非状组织相比,HHV-8 ORF 26 DNA 在状组织 (肺,淋巴结,皮肤,口腔) 中明显更为普遍.
- 与对照组相比,类似于菌细菌的16SrRNADNA在肉淋巴结中更常见,但与其他组织类型相比,其不常见.
- 检测到的HHV-8的序列是多样化的,其中有一些独特的变异,并且与已知的真菌细菌物种不相应.
结论:
- 变异的HHV-8 DNA 序列经常在各种肉体组织中发现,但在非肉体组织中没有.
- 类似于真菌细菌的序列在 sarcoid 淋巴结中更常见,但它们的存在并不证实感染特定的真菌细菌物种.
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