突触特异性,长期促进感觉到运动突触的解:在存储记忆中的局部蛋白质合成的功能
1Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
Cell
|January 15, 1998
概括
突触可塑性可以发生在神经元的单一分支中,独立于细胞体. 这种特定于分支的变化依赖于转录和局部蛋白质合成来实现新的突触生长.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 长期持续的突触可塑性传统上涉及 soma 和核.
- 突触可塑性的精确细胞位置仍在调查中.
- 已知转录对于长期记忆形成至关重要.
研究的目的:
- 为了确定是否可以将突触可塑性局部化到特定的轴突分支.
- 为了研究潜在的分支特定的突触可塑性的分子机制.
- 探索局部蛋白质合成在突触可塑性中的作用.
主要方法:
- 培养了一个单一的分叉的Aplysia感觉神经元与两个运动神经元进行突触.
- 将血清应用于一个轴突分支,以诱导长期的突触可塑性.
- 分析了突触连接和蛋白质合成中的特定分支变化.
主要成果:
- 在单个轴突分支中证明了长期的分支特定促进作用.
- 证实这种可塑性取决于CREB介导的转录.
- 观察到的新突触生长仅在使用血清素治疗的分支上.
- 表明在前突触轴突中进行局部蛋白质合成是必要的,并且由血清素刺激.
结论:
- 突触可塑性可以是一个特定于分支的现象,不仅仅取决于 soma.
- 在突触前轴突内部的局部蛋白质合成足以支持长期的突触变化.
- 这一发现重新定义了突触可塑性的细胞单元,包括局部的轴突机制.
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