两种被认为的哺乳动物昼夜调节器,mper1和mper2,对光的不同反应
U Albrecht1, Z S Sun, G Eichele
1Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA.
Cell
|January 15, 1998
概括
研究人员发现了第二个老鼠昼夜钟基因mper2,同类于Drosophila时期. mper1和mper2的表达是异步的,mper1介导着昼夜节律的光携带.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 上神经核 (SCN) 是哺乳动物中主昼夜节律的节律器.
- 循环时钟基因调节生理和行为中的日常节奏.
- 鼠标基因mper1表现出昼夜表达模式,并对光线线索做出反应.
研究的目的:
- 为了识别和表征额外的哺乳动物昼夜钟基因.
- 为了研究mper1和一个新发现的基因mper2.2之间的关系.
- 阐明mper1和mper2在昼夜节律的光学携带中的作用.
主要方法:
- 对mper2基因的分离和表征.
- 在小鼠SCN中分析mper1和mper2表达模式.
- 评估基因表达反应的光/黑暗周期和持续的黑暗.
主要成果:
- 确定了第二个小鼠昼夜钟基因mper2,与Drosophila时期有显著的相似性.
- mper1和mper2表现出重叠但异步的表达模式,差异约为4小时.
- mper1在循环时间22 (CT22) 显示出快速的光诱导表达,这种反应在mper2.2中没有观察到.
结论:
- mper1和mper2是哺乳动物昼夜时钟机制的关键组成部分.
- mper1和mper2的异步表达表明了一个复杂的监管网络.
- 似乎mper1作为主要的光响应心脏起器组件,调解着昼夜系统的光带动.
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