恩多林-1 抑制了在培养的老鼠肺内皮细胞中的内多林转化酶-1 表达
1Third Department of Internal Medicine, Kumamoto University School of Medicine, Honjo, Japan.
Circulation
|February 14, 1998
概括
恩多林-1 (ET-1) 降低了肺细胞内内多林转化酶-1 (ECE-1) 的表达. 这种反机制涉及ETB受体,影响ET-1生物合成.
科学领域:
- 肺部医学 肺部医学
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
背景情况:
- 肺部富含内甲林转化酶-1 (ECE-1),对于合成血管活性内甲林-1 (ET-1) 至关重要.
- 之前没有报告ET-1和ECE-1表达之间的关系.
研究的目的:
- 研究ET-1对培养大鼠肺内皮细胞中ECE-1表达的作用.
主要方法:
- 基因和蛋白质表达分析通过北方和西方的抹黑.
- 细胞被用ET-1,ETB受体对抗剂 (BQ788),ETA受体对抗剂 (BQ123),离子素和蛋白质激酶C激活剂进行治疗.
主要成果:
- 在6小时后,ET-1显著降低了ECE-1mRNA和蛋白质水平.
- 该效应被ETB对抗剂BQ788阻止,但ETA对抗剂BQ123.3没有阻止.
- 离子胺素模仿ET-1的效果,而蛋白激酶C激活剂则没有.
结论:
- ET-1 通过通过ETB受体抑制mRNA表达来抑制ECE-1蛋白.
- 这表明ET-1在肺内皮细胞中调节自身合成途径的反循环.
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