哈姆斯特细胞中集成的病毒基因的独特模式,由高度致癌的人类腺病毒12转化
Cell
|March 1, 1976
概括
人类腺病毒12 (Ad 12) 的DNA序列被整合到仓鼠细胞中,其中93-100%的病毒DNA存在. 这项研究提供了第一个直接证据,证明了细胞DNA中的共存链接的人类腺病毒基因.
科学领域:
- 在瘤学瘤学.
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 人类腺病毒12 (Ad 12) 是一种高度致癌的腺病毒.
- 通过Ad 12的细胞转化涉及将病毒DNA集成到宿主基因组中.
- 了解整合模式对于理解AD12诱导的瘤发生至关重要.
研究的目的:
- 分析病毒DNA序列集成到HE C19仓鼠细胞中,由Ad 12转换.
- 为Ad 12基因与细胞DNA的共价链接提供直接证据.
- 为了比较Ad12转化细胞与其他腺病毒类型的整合模式.
主要方法:
- 标记为Ad 12的DNA与HE C19细胞DNA的和杂交.
- 使用Ad 12 32P-DNA和HE C19 DNA的重组动力学.
- 对Ad 12EcoRI限制DNA片段的分析与HE C19DNA重新炼.
- 准备和分析HE C19 DNA"网络".
主要成果:
- 93-100%的Ad 12 DNA序列存在于转变的HE C19细胞中.
- 每个双胞胎细胞中检测到大约8个Ad 12DNA的病毒基因组对应物.
- 六个Ad 12EcoRI碎片中的每一个都存在于每个细胞中的8到10.5个副本,这表明它们的分布接近于等角.
- 通过DNA网络分析,可以直接证实Ad 12基因与细胞DNA的共价链接.
- 在广告12号仓鼠瘤细胞系HT-2中观察到类似的整合模式.
结论:
- 人类腺病毒12基因与转变的仓鼠细胞中的细胞DNA有共连接.
- 在Ad12转换的仓鼠细胞中集成的病毒基因的模式与C组和B组人类腺病毒和SV40的模式不同.
- 这些发现有助于了解腺病毒诱导癌症的分子机制.
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