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Sec3p是开花的酵母酵母中极化分泌的空间地标
F P Finger1, T E Hughes, P Novick
1Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8002, USA.
Cell
|March 10, 1998
概括
Sec3蛋白作为酵母细胞外的空间地标,指导囊泡融合部位. 它的局部化独立于其他细胞通路,这表明它在极化分泌中起着主要作用.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 酵母细胞外发生在动态等离子体膜子域.
- 这些外细胞位点的蛋白质决定因素目前尚不清楚.
- 了解这些决定因素对于细胞循环调节至关重要.
研究的目的:
- 为了确定酵母中异细胞形成部位的主要蛋白质决定因素.
- 研究Sec3蛋白在确定这些位点中的作用.
- 阐明细胞循环期间极化外细胞形成的机制.
主要方法:
- 用于活细胞成像的Sec3蛋白与绿色光蛋白 (Sec3-GFP) 的功能融合.
- 使用已知外细胞突变标记物 (Sec4p,Sec8p) 的局部化研究.
- 在各种细胞条件下评估Sec3-GFP局部化 (例如,破坏细胞骨,改变分泌通路).
主要成果:
- 在不同细胞周期阶段,Sec3-GFP始终局部化到极化外细胞形成的部位.
- Sec3-GFP与已建立的外细胞酶蛋白Sec4p和Sec8p结合在一起.
- Sec3-GFP局部化不受分泌途径,actin/septin细胞骨或极性建立蛋白质的影响.
结论:
- Sec3蛋白质作为一个空间地标,定义了酵母酵母细胞外的部位.
- 两极分离的分泌产生的结合了actin依赖的囊泡向和Sec3p介导的融合点的建立.
- Sec3p在组织极化外细胞形成中起着根本的上游作用.
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