子头 ribozyme 自分裂的结构基础
J B Murray1, D P Terwey, L Maloney
1Department of Chemistry, Indiana University, Bloomington 47405, USA.
Cell
|March 20, 1998
概括
研究人员捕获了一个关键的形状变化在头 ribozyme 在自我裂变. 这一发现揭示了具有催化相关的中间状态,对于理解RNA酶机制至关重要.
科学领域:
- 结构生物学 结构生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 头核糖酶是各种生物过程中必不可少的小型催化RNA.
- 了解RNA自我分裂的机制对于破译 ribozyme 功能至关重要.
研究的目的:
- 为了捕捉和描述在头核糖酶在自我分裂过程中发生在头核糖酶的分裂部位的短暂形状变化.
- 为了阐明头核糖酶介导的RNA裂变的催化相关的中间状态.
主要方法:
- 使用X射线晶体学可视化结构重组.
- 使用物理和化学捕获技术,包括使用5'-C-甲基化核糖,以稳定中间体.
- 在晶体内启动自我裂变反应允许动力捕获.
主要成果:
- 成功捕获和可视化了裂变部位的8.7 Å的形状变化.
- 被困的构造代表了一个准备过渡状态几何构造的状态.
- 经过修改的 ribozyme 允许对这种过渡状态进行晶体学观察.
- 晶体中的裂变比溶液中的裂变更快,证实了具有催化作用的相关中间体.
结论:
- 这项研究提供了第一个结晶学证据,证明了头核糖酶自我裂变中的催化相关中间体.
- 捕获的构造对于理解头 рибо酶的RNA催化精确机制至关重要.
- 这项工作提供了对RNA酶及其催化机制的动态性质的见解.
相关概念视频
Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...


